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关键词：制备
于广利1 王 群2 管华诗1 徐家敏1 Robert J. Linhardt2
(1青岛海洋大学海洋药物与食品研究所, 青岛, 266003)
(2美国爱荷华大学药物与天然产物化学系, 美国, IA 52242)
摘 要 利用肝素酶 (Heparinase I, EC 4.2.2.7) 对牛肺肝素进行控制酶解, 混合寡糖经超滤、凝胶渗透色谱和高压液相色谱技术分离制备后，得到聚合度为2，4，6，8，10，12，14 和20的寡糖纯品。各寡糖纯度采用毛细管电泳和聚丙烯酰胺凝胶电泳进行检验，寡糖结构采用核磁共振氢谱证实。
关键词 牛肺；肝素寡糖；制备
中图法分类号 R284 文章编号 1001-1862(2002)02-231-05
Preparation of Bovine Lung Heparin Oligosaccharides
Yu Guangli1 Wang Qun2 Guan Huashi1 Xu Jiamin1 Robert J. Linhardt2
(1 Institute of Marine Foods and Drugs, Ocean University
of Qingdao, Qingao 266003, China)
(2 Division of Medicinal and Natural Products Chemistry,
University of Iowa, IA 52242, USA)
Abstract The method of preparing pure disaccharide, tetrasaccharide, hexasaccharide, octasaccharide, decasaccharide, dodecasaccharide，tetradecasaccharide and eicosaccharide of bovine lung heparin by using heparinase I (EC 4.2.2.7) hydrolysis, ultra filtration, gel permeation chromatography and high performance liquid chromatography techniques was reported in this paper. The purity of each oligos was tested by capillary electrophoresis and polyacrylamide gel electrophoresis. The structure of different oligos was conformed by 1H-NMR technique.
Key words bovine lung; heparin oligosaccharide; preparation
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